Article
Stabilization of a degradable protein by its overexpression in Escherichia coli.
Gene - 1 Jan 2000
Cheng Y S, Kwoh D Y, Kwoh T J, Soltvedt B C, Zipser D
Abstract excerpt
Synthesis of proteins in Escherichia coli using recombinant DNA methodology has become an important tool for isolating and studying proteins. However, the E. coli protein degradation systems can interfere with the expression of cloned genes. To examine the effect of protein degradation, we have cloned the X90 allele of the E. coli lacZ gene. The X90 allele, an ochre mutant, codes for beta-galactosidase lacking...
Topics
- Bacterial Proteins
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Bacterial
- Escherichia coli
- Galactosidases
- Gene Expression Regulation
- Lac Operon
- Mutation
- Plasmids
- beta-Galactosidase