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Article

A method to solubilise protein aggregates for immunoassay quantification which overcomes the neurofilament "hook" effect.

Journal of neuroscience methods - 15 Feb 2011

Lu Ching-Hua, Kalmar Bernadett, Malaspina Andrea, Greensmith Linda, Petzold Axel

Abstract excerpt

INTRODUCTION: Neurofilament (Nf) aggregates are a common pathological feature of neurodegenerative disorders. Although Nf levels have been investigated as a potential disease biomarker, Nf aggregates may mask Nf epitopes, preventing accurate quantification by immunoassay. Using the SOD1(G93A) mouse model of amyotrophic lateral sclerosis, we developed a method to disrupt Nf aggregates, allowing optimal immunoassay...

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Source

Identifiers

PMID: 21134399
DOI: 10.1016/j.jneumeth.2010.11.026

Genes

SOD1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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