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Article

Construction and characterization of new host-vector systems for the enforcement-cloning method.

Gene - 31 Dec 1993

Hashimoto-Gotoh T, Tsujimura A, Kuriyama K, Matsuda S

Abstract excerpt

The Escherichia coli host strains, TH1, TH2, TH3 alpha, TH4 and TH5, all trpR-, rpsL- and supE-, were constructed to constitutively express a trp promoter/operator (POtrp)-driven synthetic rpsLam+ gene encoding the streptomycin sensitivity (Sms) determinant (ribosomal protein S12). The applicabil...

Topics

Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA, Bacterial
Escherichia coli
Escherichia coli Proteins
Genetic Vectors
Molecular Sequence Data
Operator Regions, Genetic
Phenotype
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Promoter Regions, Genetic

Restriction Mapping

Ribosomal Protein S9

Ribosomal Proteins

Source

Identifiers

PMID: 8299949
DOI: 10.1016/0378-1119(93)90008-q

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8299949-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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