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Article

Construction and characterization of new host-vector systems for the enforcement-cloning method.

Gene - 31 Dec 1993

Hashimoto-Gotoh T, Tsujimura A, Kuriyama K, Matsuda S

Abstract excerpt

The Escherichia coli host strains, TH1, TH2, TH3 alpha, TH4 and TH5, all trpR-, rpsL- and supE-, were constructed to constitutively express a trp promoter/operator (POtrp)-driven synthetic rpsLam+ gene encoding the streptomycin sensitivity (Sms) determinant (ribosomal protein S12). The applicability of these strains to the Sms-enforcement cloning procedure [Toba-Minowa and Hashimoto-Gotoh, Gene 121 (1992) 25-33]...

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Identifiers

PMID: 8299949
DOI: 10.1016/0378-1119(93)90008-q

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