A radioactive assay for the physiological activity of the tryptophan synthetase alpha subunit in crude extracts of Escherichia coli.

doi:10.1016/0300-9084(90)90005-2

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Article

A radioactive assay for the physiological activity of the tryptophan synthetase alpha subunit in crude extracts of Escherichia coli.

Biochimie - 1 Dec 1990

Sörensen P M, Hijazi K A, Murgola E J, Buckingham R H

Abstract excerpt

A modified assay has been devised for the physiological reaction, indole-3-glycerol phosphate to Trp, of the enzyme tryptophan synthetase. The assay may be applied to crude bacterial extracts, and is based on the measurement of incorporation of radioactivity from [3H]Ser into Trp. Comparison with...

Topics

Colorimetry
Escherichia coli
Evaluation Studies as Topic
Mutation
Protein Conformation
Tritium
Tryptophan Synthase

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