Article
Estimation of D segment usage in initial D to JH joinings in a murine immature B cell line. Preferential usage of DFL16.1, the most 5' D segment and DQ52, the most JH-proximal D segment.
Journal of immunology (Baltimore, Md. : 1950) - 15 May 1990
Tsukada S, Sugiyama H, Oka Y, Kishimoto S
Abstract excerpt
We established SPL2-1-2, a murine immature B cell line transformed by tsOS-59, a temperature-sensitive mutant of Abelson murine leukemia virus. SPL2-1-2 has a VHDJH-/G (germ-line) configuration, and can continuously generate D to JH joinings from the germ-line allele during culture. The D to JH j...
Topics
- Alleles
- Animals
- B-Lymphocytes
- Blotting, Southern
- Cell Differentiation
- Chromosome Deletion
- DNA Mutational Analysis
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Genes, Immunoglobulin
- Immunoglobulin Heavy Chains
- In Vitro Techniques
- Mice
- Recombination, Genetic
- Temperature