Biogenesis of vacuolar membrane glycoproteins of yeast Saccharomyces cerevisiae.

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Article

Biogenesis of vacuolar membrane glycoproteins of yeast Saccharomyces cerevisiae.

The Journal of biological chemistry - 5 May 1990

Nishikawa S, Umemoto N, Ohsumi Y, Nakano A, Anraku Y

Abstract excerpt

To investigate the biogenesis of the yeast vacuole, we have sought novel marker proteins localized to the vacuolar membrane. Glycoproteins were prepared from vacuolar membrane vesicles by concanavalin A-Sepharose column chromatography and used to raise monoclonal antibodies. The antibodies obtain...

Topics

Acetylglucosaminidase
Antibodies, Monoclonal
Cell Fractionation
Centrifugation, Density Gradient
Fluorescent Antibody Technique
Genotype
Intracellular Membranes
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Membrane Glycoproteins
Molecular Weight
Saccharomyces cerevisiae
Vacuoles

Source

Open in PubMed

Identifiers

PMID: 2110166

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2110166-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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