Article
Mechanism of protein splicing of the Pyrococcus abyssi lon protease intein.
Biochemical and biophysical research communications - 17 Dec 2010
O'Brien Kevin M, Schufreider Ann K, McGill Melissa A, O'Brien Kathryn M, Reitter Julie N, Mills Kenneth V
Abstract excerpt
Protein splicing is a post-translational process by which an intervening polypeptide, the intein, excises itself from the flanking polypeptides, the exteins, coupled to ligation of the exteins. The lon protease of Pyrococcus abyssi (Pab) is interrupted by an intein. When over-expressed as a fusion protein in Escherichia coli, the Pab lon protease intein can promote efficient protein splicing. Mutations that block...
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