Article
Rescue of protein splicing activity from a Magnetospirillum magnetotacticum intein-like element.
Biochemical Society transactions - 1 Apr 2004
Southworth M W, Yin J, Perler F B
Abstract excerpt
The self-catalytic protein splicing mechanism is mediated by the intein plus the first amino acid following the intein C-terminus (termed the +1 residue). Although polymorphisms of conserved residues elsewhere in inteins have been widely reported, no splicing-competent intein has been observed without a Ser, Thr or Cys in this functionally essential +1 position. This residue is the nucleophile in two steps of the...
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