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Article

Heterologous expression and characterization of mutant cellulase from indigenous strain of Aspergillus niger.

PloS one - 1 Jan 2024

Ahmad Waqas, Zafar Muddassar, Anwar Zahid

Abstract excerpt

The purpose of current research work was to investigate the effect of mutagenesis on endoglucanase B activity of indigenous strain of Aspergillus niger and its heterologous expression studies in the pET28a+ vector. The physical and chemical mutagens were employed to incorporate mutations in A. niger. For determination of mutations, mRNA was isolated followed by cDNA synthesis and cellulase gene was amplified,...

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Identifiers

PMID: 38748703
DOI: 10.1371/journal.pone.0298716
PMCID: PMC11095671

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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