Article
Transformation of Aspergillus flavus: construction of urate oxidase-deficient mutants by gene disruption.
Current genetics - 1 May 1992
Chevalet L, Tiraby G, Cabane B, Loison G
Abstract excerpt
A transformation procedure based on the complementation of a genetic defect was developed using a nitrate reductase-deficient mutant of Aspergillus flavus. The initial transformation efficiency was improved 40-fold by combining factors in a planned experimental program. Although low, this transfo...
Topics
- Aspergillus flavus
- Blotting, Southern
- Cloning, Molecular
- Genetic Complementation Test
- Mutation
- Nitrate Reductase
- Nitrate Reductases
- Plasmids
- Restriction Mapping
- Transformation, Genetic
- Urate Oxidase
- Uric Acid