Article
Transformation of Aspergillus flavus: construction of urate oxidase-deficient mutants by gene disruption.
Current genetics - 1 May 1992
Chevalet L, Tiraby G, Cabane B, Loison G
Abstract excerpt
A transformation procedure based on the complementation of a genetic defect was developed using a nitrate reductase-deficient mutant of Aspergillus flavus. The initial transformation efficiency was improved 40-fold by combining factors in a planned experimental program. Although low, this transformation rate was sufficient to obtain transformants in which the urate oxidase-encoding gene (uaZ) was disrupted in a...
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