Article
Antibody domain mutants demonstrate autonomy of the antigen binding site.
The EMBO journal - 1 Apr 1990
Simon T, Rajewsky K
Abstract excerpt
We have constructed derivatives of a lambda I light chain-bearing anti-(4-hydroxy,3-nitrophenyl)acetyl (NP) antibody which have the V regions exchanged between heavy chains and light chains of the kappa or lambda I type. These antibodies are assembled and secreted normally, and bind haptenic and...
Topics
- Animals
- Antigen-Antibody Complex
- Binding Sites, Antibody
- Cell Line
- Haptens
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains
- Immunoglobulin Variable Region
- Immunoglobulin lambda-Chains
- Kinetics
- Mice
- Multiple Myeloma
- Mutation
- Plasmids
- Recombination, Genetic
- Transfection