Article
A dominant negative mutation in two proteins created by ectopic expression of an AU-rich 3' untranslated region.
Somatic cell and molecular genetics - 1 Mar 1990
Bunnell B A, Fillmore H, Gregory P, Kidd V J
Abstract excerpt
We have found that the ectopic expression of a specific gene's 3' untranslated region leads to the dose dependent loss, relative to gene copy number, of that specific mRNA and protein, as well as an associated protein, in a eukaryotic cell line. The loss of these proteins from the eukaryotic cell...
Topics
- Adenine
- Base Sequence
- Cell Line
- DNA
- Fluorescent Antibody Technique
- Galactosyltransferases
- Gene Amplification
- Gene Expression
- Genes, Dominant
- Genes, Regulator
- Humans
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Phenotype
- Protein Biosynthesis
- Protein Kinases