Article
Generation of genetically modified rodents using random ENU mutagenesis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
van Boxtel Ruben, Cuppen Edwin
Abstract excerpt
The generation of genetically modified animals using N-ethyl-N-nitrosourea (ENU) mutagenesis is a fast and highly effective method. The technique is based on treating male animals with the supermutagen ENU, which randomly introduces mutations in the spermatogonial stem cells. By breeding these animals with untreated females, an F1 population is generated in which each individual carries unique random ENU-induced...
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