Article
Qualitative and quantitative determination of quorum sensing inhibition in vitro.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Jakobsen Tim Holm, van Gennip Maria, Christensen Louise Dahl, Bjarnsholt Thomas, Givskov Michael
Abstract excerpt
The formation of biofilms in conjunction with quorum sensing (QS)-regulated expression of virulence by opportunistic pathogens contributes significantly to immune evasion and tolerance to a variety of antimicrobial treatments. The present protocol describes methods to determine the in vitro efficacy of potential quorum sensing inhibitors (QSIs). Work on Pseudomonas aeruginosa has shown that chemical blockage of...
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