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Generation of stable, high‐producing cho cell lines by lentiviral vector‐mediated gene transfer in serum‐free suspension culture

21 Oct 2010

Abstract excerpt

Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell sorting (FACS). For comparison, cell pools and cell lines were also generated by transfection, using the LV transfer plasmid alone. The level and stability of...

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PMID: 20967750
DOI: 10.1002/bit.22968

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