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Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct

14 Aug 2020

Abstract excerpt

Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient transfection of adherent 293T cells with multiple plasmids. We report here a method to rapidly generate such producer cell lines from 293T cells by stable transfection of a...

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PMID: 32995359
DOI: 10.1016/j.omtm.2020.08.011

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