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Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines

30 Nov 2015

Abstract excerpt

Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposons-piggyBac, Tol2, and Sleeping Beauty-for their ability to generate cell pools (polyclonal cultures of recombinant cells) and clonal cell lines for the large-scale production of recombinant proteins using Chinese hamster ovary...

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PMID: 26616356
DOI: 10.1002/bit.25888

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