Article
Clonal tracking of hESCs reveals differential contribution to functional assays.
Nature methods - 1 Nov 2010
Stewart Morag H, Bendall Sean C, Levadoux-Martin Marilyne, Bhatia Mickie
Abstract excerpt
Human embryonic stem cells (hESCs) have unique self-renewal and differentiation properties, which are experimentally measured using functional assays. hESC cultures are known to be heterogeneous, but whether subsets of cells contribute differently to functional assays has yet to be examined. Here, using clonal tracking by retroviral integration, we analyzed in situ the propensity of individual hESCs to contribute...
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