Article
Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro.
Stem cell research - 1 Sept 2014
Ovchinnikov Dmitry A, Titmarsh Drew M, Fortuna Patrick R J, Hidalgo Alejandro, Alharbi Samah, Whitworth Deanne J, Cooper-White Justin J, Wolvetang Ernst J
Abstract excerpt
Optimization of pluripotent stem cell expansion and differentiation is facilitated by biological tools that permit non-invasive and dynamic monitoring of pluripotency, and the ability to select for an undifferentiated input cell population. Here we report on the generation and characterisation of clonal human embryonic stem (HES3, H9) and human induced pluripotent stem cell lines (UQEW01i-epifibC11) that have...
Topics
- Anti-Bacterial Agents
- Bioreactors
- Cell Culture Techniques
- Cell Differentiation
- Cell Line
- Cell Proliferation
- Drug Resistance, Bacterial
- Embryonic Stem Cells
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Genetic Markers
- Genetic Vectors
