Article
Strand transfer and elongation of HIV-1 reverse transcription is facilitated by cell factors in vitro.
PloS one - 6 Oct 2010
Warrilow David, Warren Kylie, Harrich David
Abstract excerpt
Recent work suggests a role for multiple host factors in facilitating HIV-1 reverse transcription. Previously, we identified a cellular activity which increases the efficiency of HIV-1 reverse transcription in vitro. Here, we describe aspects of the activity which shed light on its function. The cellular factor did not affect synthesis of strong-stop DNA but did improve downstream DNA synthesis. The stimulatory...
Topics
- Capsid
- Deoxyribonuclease I
- HIV Reverse Transcriptase
- HIV-1
- Humans
- In Vitro Techniques
- Jurkat Cells
- Mutation
- Octoxynol
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic
- Virion