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Identification of Murine Rotavirus Virulence Determinants Using Bidirectional Selective Passaging and a Reverse Genetics System.

Viruses - 6 Jul 2026

Fukuda Saori, Kugita Masanori, Akari Yuki, Dijkstra Johannes M, Kawamura Yoshiki, Nagao Shizuko, Yoshikawa Tetsushi, Murata Takayuki, Komoto Satoshi

Abstract excerpt

Live-attenuated rotavirus (RV) vaccines are the most effective interventions for preventing RV gastroenteritis (RVGE) in young children. However, the molecular basis of attenuation remains not well understood. Here, we describe a compact but comprehensive strategy to identify RV virulence determinants by combining low-passage bidirectional selection, sequence analysis, and segment-level phenotype testing via a...

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Identifiers

PMID: 42515599
DOI: 10.3390/v18070747
PMCID: PMC13431595

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