Article
Quantitative effects of magnesium chloride stress on aggregation of Sup35p in [psi-] yeast cells.
Protein and peptide letters - 1 Dec 2010
Song Yao, Lan Wanjun, Wu Xianyuan, He Jianwei, Li Hui, Ben Songbin, Song Youtao
Abstract excerpt
[PSI(+)] phenotype can be transiently induced when Magnesium chloride (MgCl(2)) was the selective pressure in SUP35 repeat-expansion mutant [psi(-)] yeast strains. We further investigated [PSI(+)] phenotype change under different MgCl(2) conditions with native Sup35p and quantified the Sup35p status changes with fluorescence recovery after photobleaching (FRAP) and semi-denaturing detergent-agarose gel...
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