Article
Generation of representative primary virus isolates from blood plasma after isolation of HIV-1 with CD44 MicroBeads.
Archives of virology - 1 Dec 2010
Cornelissen Marion, Heeregrave Edwin J, Zorgdrager Fokla, Pollakis Georgios, Paxton William A, van der Kuyl Antoinette C
Abstract excerpt
Infection of cell cultures with cell-free virus isolated from HIV-infected patients is notoriously difficult and results in a loss of viral variation. Here, we describe viral sequences from PBMC, U87.CD4.CCR5 and U87.CD4.CXCR4 cell cultures and compare them to those from blood plasma from 12 pati...
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