Article
Selection of HIV-1 genotypes by cultivation in different primary cells.
AIDS (London, England) - 25 Feb 1999
von Briesen H, Grez M, Ruppach H, Raudonat I, Unger R E, Becker K, Panhans B, Dietrich U, Rübsamen-Waigmann H
Abstract excerpt
OBJECTIVES: To determine the representation of particular HIV-1 genotypes during cultivation in different primary cell-culture systems compared with the spectrum of the quasispecies in vivo. METHODS: Primary isolates of HIV-1 were recovered by isolation in cultures of lymphocytes, mixed mononuclear cells (MNC), and monocytes/macrophages. Nucleotide sequence determination of the C2-V3 region of gp120 of HIV was...
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