Article
Determination of coreceptor usage of human immunodeficiency virus type 1 from patient plasma samples by using a recombinant phenotypic assay.
Journal of virology - 1 Jan 2001
Trouplin V, Salvatori F, Cappello F, Obry V, Brelot A, Heveker N, Alizon M, Scarlatti G, Clavel F, Mammano F
Abstract excerpt
We developed a recombinant virus technique to determine the coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from plasma samples, the source expected to represent the most actively replicating virus population in infected subjects. This method is not subject to selective bias associated with virus isolation in culture, a step required for conventional tropism determination procedures. The addition...
Topics
- Acquired Immunodeficiency Syndrome
- Amino Acid Sequence
- Cells, Cultured
- Genetic Vectors
- HIV-1
- Humans
- Molecular Sequence Data
- Phenotype
- Receptors, CCR5
- Receptors, CXCR4
- Recombination, Genetic
- Viremia
- Virus Replication