Article
Trapping and spectroscopic characterization of an FeIII-superoxo intermediate from a nonheme mononuclear iron-containing enzyme.
Proceedings of the National Academy of Sciences of the United States of America - 28 Sept 2010
Mbughuni Michael M, Chakrabarti Mrinmoy, Hayden Joshua A, Bominaar Emile L, Hendrich Michael P, Münck Eckard, Lipscomb John D
Abstract excerpt
Fe(III)-O(2)*(-) intermediates are well known in heme enzymes, but none have been characterized in the nonheme mononuclear Fe(II) enzyme family. Many steps in the O(2) activation and reaction cycle of Fe(II)-containing homoprotocatechuate 2,3-dioxygenase are made detectable by using the alternative substrate 4-nitrocatechol (4NC) and mutation of the active site His200 to Asn (H200N). Here, the first intermediate...
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