Development of a Genetic System for Combinatorial Biosynthesis of Lipopeptides in <i>Streptomyces fradiae</i> and Heterologous Expression of the A54145 Biosynthesis Gene Cluster

Abstract excerpt

A54145 factors are calcium-dependent lipopeptide antibiotics produced by Streptomyces fradiae NRRL 18160. A54145 is structurally related to the clinically important daptomycin, and as such may be a useful scaffold for the development of a novel lipopeptide antibiotic. We developed methods to genetically manipulate S. fradiae by deletion mutagenesis and conjugal transfer of plasmids from Escherichia coli. Cloning...

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