Article
A split active site couples cap recognition by Dcp2 to activation.
Nature structural & molecular biology - 1 Sept 2010
Floor Stephen N, Jones Brittnee N, Hernandez Gail A, Gross John D
Abstract excerpt
Decapping by Dcp2 is an essential step in 5'-to-3' mRNA decay. In yeast, decapping requires an open-to-closed transition in Dcp2, though the link between closure and catalysis remains elusive. Here we show using NMR that cap binds conserved residues on both the catalytic and regulatory domains of Dcp2. Lesions in the cap-binding site on the regulatory domain reduce the catalytic step by two orders of magnitude...
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