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Article

Characterization of a temperature-sensitive vertebrate clathrin heavy chain mutant as a tool to study clathrin-dependent events in vivo.

PloS one - 6 Aug 2010

Neumann-Staubitz Petra, Hall Stephanie L, Kuo Joseph, Jackson Antony P

Abstract excerpt

Clathrin and clathrin-dependent events are evolutionary conserved although it is believed that there are differences in the requirement for clathrin in yeast and higher vertebrates. Clathrin is a long-lived protein and thus, with clathrin knockdowns only long-term consequences of clathrin depletion can be studied. Here, we characterize the first vertebrate temperature-sensitive clathrin heavy chain mutant as a...

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Identifiers

PMID: 20700507
DOI: 10.1371/journal.pone.0012017
PMCID: PMC2917355

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