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Article

Extensive recombination due to heteroduplexes generates large amounts of artificial gene fragments during PCR.

PloS one - 1 Jan 2014

Liu Jia, Song Hongshuo, Liu Donglai, Zuo Tao, Lu Fengmin, Zhuang Hui, Gao Feng

Abstract excerpt

Artificial recombinants can be generated during PCR when more than two genetically distinct templates coexist in a single PCR reaction. These recombinant amplicons can lead to the false interpretation of genetic diversity and incorrect identification of biological phenotypes that do not exist in vivo. We investigated how recombination between 2 or 35 genetically distinct HIV-1 genomes was affected by different...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25211143
DOI: 10.1371/journal.pone.0106658
PMCID: PMC4161356

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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