Article

Long Range PCR-based deep sequencing for haplotype determination in mixed HCMV infections

2021-07-05

Abstract excerpt

Short read sequencing, which has extensively been used to decipher the genome diversity of human cytomegalovirus (HCMV) strains, often falls short to assess co-linearity of non-adjacent polymorphic sites in mixed HCMV populations. In the present study, we established a long amplicon sequencing workflow to identify number and relative quantities of unique HCMV haplotypes in mixtures. Accordingly, long read PacBio s...

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Literature Corpus work: 74e5c569-dc8f-52f5-814f-edebda61b84a
DOI: 10.1101/2021.07.05.451103

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