Article
Reducing chimera formation during PCR amplification to ensure accurate genotyping.
Gene - 1 Dec 2010
Smyth R P, Schlub T E, Grimm A, Venturi V, Chopra A, Mallal S, Davenport M P, Mak J
Abstract excerpt
Measurements of population diversity are fundamental to the reconstruction of the evolutionary and epidemiological history of organisms. Commonly used protocols to measure population diversity using the polymerase chain reaction (PCR) are prone to the introduction of artificial chimeras. These are often difficult to detect and can confound the correct interpretation of results due to the false generation of...
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