Article
Disposable sensors for rapid screening of mutated genes.
Analytical and bioanalytical chemistry - 1 Oct 2010
García T, Fernández-Barrena M G, Revenga-Parra M, Núñez A, Casero E, Pariente F, Prieto J, Lorenzo E
Abstract excerpt
A screening method for rapid detection of gene mutations directly in polymerase chain reaction (PCR) of genomic DNA is described. The method involves the development of a disposable screen-printed gold electrode modified with a thiolated capture probe directly obtained from denaturated PCR genomic DNA, which recognizes (by hybridization) its fully complementary sequence (wild type), giving a signal, whereas no...
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