Article
Rapid, sensitive, and label-free impedimetric detection of a single-nucleotide polymorphism correlated to kidney disease.
Analytical chemistry - 1 May 2010
Bonanni Alessandra, Pumera Martin, Miyahara Yuji
Abstract excerpt
We present a protocol for the very rapid and sensitive detection of a specific mutation of the COL4A5 gene (exon 29, A-C mismatch) which was found in people affected by Alport syndrome (AS) and their families. Disposable electrochemically printed electrodes were used to immobilize a single-stranded oligonucleotide probe that was complementary to the AS-correlated gene. The detection principle is based on changes...
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