Article
Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.
Molecular and cellular probes - 1 Dec 2010
Peruzzi Benedetta, Serra Martina, Pescucci Chiara, Sica Michela, Lastraioli Sonia, Rondelli Tommaso, Pedemonte Simona, Risitano Antonio Maria, De Angioletti Maria, Piccioli Patrizia, Notaro Rosario
Abstract excerpt
Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.
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