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Evaluation of a new efficient procedure for single-nucleotide polymorphism genotyping: tetra-primer amplification refractory mutation system-polymerase chain reaction.

Clinical chemistry and laboratory medicine - 1 Jan 2004

Okayama Naoko, Fujimura Kozue, Nakamura Junji, Suehiro Yutaka, Hamanaka Yuichiro, Hinoda Yuji

Abstract excerpt

Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) is a new efficient method for single-nucleotide polymorphism (SNP) genotyping. To determine the optimal conditions for ARMS-PCR we attempted to genotype ten SNPs. DNA was extracted from the peripheral blood of 168 unrelated healthy Japanese volunteers. Two problems inhibited uniform efficiency of the amplification of three...

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Asian People
DNA Primers
Genotype
Japan
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Polymorphism, Single Nucleotide

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PMID: 15061374
DOI: 10.1515/CCLM.2004.004

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