Article
A chemostat array enables the spatio-temporal analysis of the yeast proteome.
Proceedings of the National Academy of Sciences of the United States of America - 24 Sept 2013
Dénervaud Nicolas, Becker Johannes, Delgado-Gonzalo Ricard, Damay Pascal, Rajkumar Arun S, Unser Michael, Shore David, Naef Felix, Maerkl Sebastian J
Abstract excerpt
Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min time resolution. We measured protein abundance and localization changes in 4,085 GFP-tagged strains in response to methyl methanesulfonate and analyzed 576 GFP...
Topics
- Gene Deletion
- Green Fluorescent Proteins
- Methyl Methanesulfonate
- Microfluidics
- Phenotype
- Proteome
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Spatio-Temporal Analysis