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Article

An investigation of Cu2Zn2 superoxide dismutase and its Ile-137 mutant at high pH.

European biophysics journal : EBJ - 1 Jan 1991

Banci L, Bertini I, Turano P

Abstract excerpt

The activity profile of the Cu2Zn2HSOD Ile-137 mutant has a pKa of 9.6, i.e. one unit lower than the wild type (WT). This property has allowed us to investigate the inactive high pH form of the enzyme before denaturation occurs. The electronic and EPR spectra do not change with the above pKa. The 1H NMR spectrum of the Cu2Co2-analog reveals slight decreases in the hyperfine shifts of the protons of His-48 at high...

Topics

Azides
Circular Dichroism
Cobalt
Copper
Histidine
Humans
Hydrogen-Ion Concentration
Isoleucine
Kinetics
Magnetic Resonance Spectroscopy
Mutation
Spectrophotometry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Superoxide Dismutase

Water

Zinc

Source

Identifiers

PMID: 2060493
DOI: 10.1007/BF00185454

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2060493-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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