Article
Effect of lysine ionization on the structure and electrochemical behaviour of the Met44-->Lys mutant of the blue-copper protein azurin from Pseudomonas aeruginosa.
European journal of biochemistry - 15 Nov 1993
Van de Kamp M, Canters G W, Andrew C R, Sanders-Loehr J, Bender C J, Peisach J
Abstract excerpt
The structural and spectrochemical effects of the replacement of Met44 in the hydrophobic surface patch of azurin from Pseudomonas aeruginosa by a lysine residue were studied as a function of the ionization state of the lysine. In the pH range 5-8, the optical absorption, resonance Raman, EPR and...
Topics
- Azurin
- Electrochemistry
- Histidine
- Ions
- Lysine
- Methionine
- Mutation
- Oxidation-Reduction
- Protein Conformation
- Pseudomonas aeruginosa
- Spectrum Analysis