Article
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat.
BMC plant biology - 24 Jun 2010
Liu Li, Ikeda Tatsuya M, Branlard Gerard, Peña Roberto J, Rogers William J, Lerner Silvia E, Kolman María A, Xia Xianchun, Wang Linhai, Ma Wujun, Appels Rudi, Yoshida Hisashi, Wang Aili, Yan Yueming, He Zhonghu
Abstract excerpt
BACKGROUND: Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF x SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
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