Article
SREBP isoform and SREBP target gene expression during rat primary hepatocyte culture.
In vitro cellular & developmental biology. Animal - 1 Sept 2010
Wu Jiakai, Dickson Alan J
Abstract excerpt
Expression of mRNA encoding sterol regulatory element binding protein (SREBP) isoforms (SREBP-1a, -1c, -2) and seven SREBP target genes decreased dramatically as a result of isolation and subsequent culture of primary rat hepatocytes. In standard maintenance medium (MM) expression remained low but when cultured in HepatoZYME (HZM), there was a selective increase in mRNA encoding SREBP-2 and a subset of SREBP...
Topics
- Animals
- CCAAT-Binding Factor
- Cell Culture Techniques
- Gene Expression
- Hepatocyte Nuclear Factor 4
- Hepatocytes
- Liver X Receptors
- Male
- Orphan Nuclear Receptors
- Phenotype
- Promoter Regions, Genetic