Article
Activities of a mechanosensitive ion channel in an E. coli mutant lacking the major lipoprotein.
The Journal of membrane biology - 1 Feb 1993
Kubalski A, Martinac B, Ling K Y, Adler J, Kung C
Abstract excerpt
The activity of the mechanosensitive (MS) ion channels in membrane patches, excised from E. coli spheroplasts, was analyzed using the patch-clamp technique. Outer membranes from a mutant lacking the major lipoprotein (Lpp) and its wild-type parent were examined. The MS-channel activities in the wild-type membrane rarely revealed substates at the time resolution used. These channels showed a stretch sensitivity...
Topics
- Animals
- Bacterial Outer Membrane Proteins
- Chlorpromazine
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Ion Channel Gating
- Ion Channels
- Lipoproteins
- Lysophosphatidylcholines
- Mutation
- Picrates