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Article

Identification of germline competent chimaeras by copulatory plug genotyping.

Transgenic research - 1 Apr 2011

Wilson Stephen, Sheardown Steven A

Abstract excerpt

The increasing use of ES cell lines from strains other than 129, in particular C57BL/6, has greatly reduced the time taken to generate gene knockouts on a defined genetic background. Generally, C57BL/6 ES cell lines transmit less efficiently through the germline than 129 lines; consequently the burden on animal husbandry at this stage is increased. Genotyping sperm from chimaeric males may be used to identify...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 20549345
DOI: 10.1007/s11248-010-9413-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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