Article
Identification of germline competent chimaeras by copulatory plug genotyping.
Transgenic research - 1 Apr 2011
Wilson Stephen, Sheardown Steven A
Abstract excerpt
The increasing use of ES cell lines from strains other than 129, in particular C57BL/6, has greatly reduced the time taken to generate gene knockouts on a defined genetic background. Generally, C57BL/6 ES cell lines transmit less efficiently through the germline than 129 lines; consequently the burden on animal husbandry at this stage is increased. Genotyping sperm from chimaeric males may be used to identify...
Topics
- Animals
- Breeding
- Cell Line
- Chimera
- Copulation
- Embryo, Mammalian
- Embryonic Stem Cells
- Female
- Gene Targeting
- Genotype
- Germ Cells
- Male