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Article

The perfect host: a mouse host embryo facilitating more efficient germ line transmission of genetically modified embryonic stem cells.

PloS one - 1 Jan 2013

Taft Robert A, Low Benjamin E, Byers Shannon L, Murray Stephen A, Kutny Peter, Wiles Michael V

Abstract excerpt

There is a continual need to improve efficiency in creating precise genetic modifications in mice using embryonic stem cells (ESCs). We describe a novel approach resulting in 100% germline transmission from competent injected ESCs. We developed an F1 mouse host embryo (Perfect Host, PH) that selectively ablates its own germ cells via tissue-specific induction of diphtheria toxin. This approach allows competent...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 23844102
DOI: 10.1371/journal.pone.0067826
PMCID: PMC3699516

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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