Article
Protein refolding is required for assembly of the type three secretion needle.
Nature structural & molecular biology - 1 Jul 2010
Poyraz Omer, Schmidt Holger, Seidel Karsten, Delissen Friedmar, Ader Christian, Tenenboim Hezi, Goosmann Christian, Laube Britta, Thünemann Andreas F, Zychlinsky Arturo, Baldus Marc, Lange Adam, Griesinger Christian, Kolbe Michael
Abstract excerpt
Pathogenic Gram-negative bacteria use a type three secretion system (TTSS) to deliver virulence factors into host cells. Although the order in which proteins incorporate into the growing TTSS is well described, the underlying assembly mechanisms are still unclear. Here we show that the TTSS needle protomer refolds spontaneously to extend the needle from the distal end. We developed a functional mutant of the...
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