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Article

Application of an inducible system to engineer unmarked conditional mutants of essential genes of Pseudomonas aeruginosa.

Journal of microbiological methods - 1 Sept 2010

Morita Yuji, Narita Shin-ichiro, Tomida Junko, Tokuda Hajime, Kawamura Yoshiaki

Abstract excerpt

The Phi CTX-based integration vector pYM101 harboring a tightly controlled modified phage T7 early gene promoter/LacI(q) repressor (T7/LacI) system was constructed for the generation of unmarked conditional mutants in Pseudomonas aeruginosa. Promoter activity of the T7/LacI system was demonstrate...

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Share to Bacterial Proteins
Share to Gene Expression Regulation, Bacterial
Share to Genes, Essential

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Mutation

Share to Promoter Regions, Genetic

Share to Pseudomonas aeruginosa

Source

Identifiers

PMID: 20538017
DOI: 10.1016/j.mimet.2010.06.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20538017-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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