Article
The FinR-regulated essential gene fprA, encoding ferredoxin NADP+ reductase: Roles in superoxide-mediated stress protection and virulence of Pseudomonas aeruginosa.
PloS one - 1 Jan 2017
Boonma Siriwan, Romsang Adisak, Duang-Nkern Jintana, Atichartpongkul Sopapan, Trinachartvanit Wachareeporn, Vattanaviboon Paiboon, Mongkolsuk Skorn
Abstract excerpt
Pseudomonas aeruginosa has two genes encoding ferredoxin NADP(+) reductases, denoted fprA and fprB. We show here that P. aeruginosa fprA is an essential gene. However, the ΔfprA mutant could only be successfully constructed in PAO1 strains containing an extra copy of fprA on a mini-Tn7 vector integrated into the chromosome or carrying it on a temperature-sensitive plasmid. The strain containing an extra copy of...
Topics
- Animals
- Bacterial Proteins
- Drosophila
- Ferredoxin-NADP Reductase
- Ferredoxins
- Gene Expression Regulation, Bacterial
- Oxidative Stress
- Phenotype
- Pseudomonas aeruginosa
- Superoxides
- Transcription Factors
- Virulence