Article
RNA interference by feeding in vitro-synthesized double-stranded RNA to planarians: methodology and dynamics.
Developmental dynamics : an official publication of the American Association of Anatomists - 1 Jun 2013
Rouhana Labib, Weiss Jennifer A, Forsthoefel David J, Lee Hayoung, King Ryan S, Inoue Takeshi, Shibata Norito, Agata Kiyokazu, Newmark Phillip A
Abstract excerpt
BACKGROUND: The ability to assess gene function is essential for understanding biological processes. Currently, RNA interference (RNAi) is the only technique available to assess gene function in planarians, in which it has been induced by means of injection of double-stranded RNA (dsRNA), soaking, or ingestion of bacteria expressing dsRNA. RESULTS: We describe a simple and robust RNAi protocol, involving in vitro...
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