Article
Applications of high-throughput RNA interference screens to problems in cell and developmental biology.
Genetics - 1 Jan 2007
Perrimon Norbert, Mathey-Prevot Bernard
Abstract excerpt
RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the...
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