Article
Calcium-dependent conformational changes in inositol trisphosphate receptors.
The Journal of biological chemistry - 6 Aug 2010
Anyatonwu Georgia, Khan M Tariq, Schug Zachary T, da Fonseca Paula C A, Morris Edward P, Joseph Suresh K
Abstract excerpt
We have used limited trypsin digestion and reactivity with PEG-maleimides (MPEG) to study Ca(2+)-induced conformational changes of IP(3)Rs in their native membrane environment. We found that Ca(2+) decreased the formation of the 95-kDa C-terminal tryptic fragment when detected by an Ab directed at a C-terminal epitope (CT-1) but not with an Ab recognizing a protected intraluminal epitope. This suggests that...
Topics
- Animals
- Binding Sites
- COS Cells
- Calcium
- Chlorocebus aethiops
- Cysteine
- Epitopes
- Inositol 1,4,5-Trisphosphate Receptors
- Microsomes, Liver
- Models, Biological
- Mutation